Authors : H. A. Nimkar, Ugale Ugale M.R.*, Dhore A.O.
Page Nos : 100-113
Description :
Fusarium wilt, caused by Fusarium oxysporum f. sp. glycines, is one of threat to soybean (Glycine max). This
study investigates the biocontrol potential of indigenous Trichoderma spp. formulated with different carrier
materials, focusing on talc-based formulations for their efficacy under pot culture conditions. From thirty
rhizospheric soil samples, twenty-five Trichoderma isolates were obtained, and three superior antagonistic
strains (TR2, TR3, and TR23) were selected based on dual culture assays. These isolates were massmultiplied
using six carrier substrates (talc, lignite, charcoal, sawdust, compost, and fly ash), and their shelf
life was evaluated over 180 days. Talc emerged as the most effective carrier, maintaining the highest colonyforming
units (CFU/g) across all time intervals. The results suggest that talc-based formulations of native
Trichoderma isolates, particularly TR2, offer a sustainable and eco-friendly alternative to chemical fungicides.
